Adult_Exposed_Male_2 - SRA

SRX2900163: Adult_Exposed_Male_2
1 ILLUMINA (Illumina HiSeq 2000) run: 24.7M spots, 4.9G bases, 1.8Gb downloads

Design: Libraries were generated from 250 ng of total RNA using the TruSeq stranded mRNA Sample Preparation Kit (Illumina), as per the manufacturer’s recommendations. Libraries were quantified using the Quant-iT™ PicoGreen® dsDNA Assay Kit (Life Technologies) and the Kapa Illumina GA with Revised Primers-SYBR Fast Universal kit (Kapa Biosystems). Average size fragment was determined using a LabChip GX (PerkinElmer) instrument. The libraries were normalized, denatured in 0.05N NaOH and then were diluted to 8pM using HT1 buffer. The clustering was done on a Illumina cBot and the flowcell was ran on a HiSeq 2000 for 2x100 cycles following the manufacturer's instructions. A phiX library was used as a control and mixed with libraries at 1% level. The Illumina control software was HCS 2.2.58, the real-time analysis program was RTA v. 1.18.63. Program bcl2fastq v1.8.4 was then used to demultiplex samples and generate fastq reads.

Submitted by: University of Perpignan

Study: Schistosoma mansoni strain LE - Transcriptome or Gene expression

PRJNA389649 • SRP108901 • All experiments • All runs

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RNA-Seq analyses for Schistosoma mansoni strain SmLE

Sample: Schistosoma mansoni SmLE adult male with latex 2

SAMN07204408 • SRS2267407 • All experiments • All runs

Organism: Schistosoma mansoni

Library:

Name: E2male

Instrument: Illumina HiSeq 2000

Strategy: RNA-Seq